Cladosporium protease/doxorubicin decorated Fe3O4@SiO2 nanocomposite: An efficient nanoparticle for drug delivery and combating breast cancer, Journal of Drug Delivery Science and Technology Volume 80, February 2023, 104144

Abstract

Fe₃O₄/SiO₂ nanocomposite was synthesized and decorated with an extracellular Cladosporium protease, doxorubicin, plus C. protease/DOX molecules. The C. Protease was used for the first time to examine its effect alone and in combination with DOX to investigate an effective as well as low-cost method to combat breast cancer by focusing the decorated magnetic nanoparticle via an external magnetic field. The C. Protease was purified by the mAzo method and the molecular weight was determined 70 kDa by SDS-PAGE. The phylogeny of Cladosporium was determined by sequencing the ITS region. The nanocomposite was characterized by FESEM, TEM, XRD, FT-IR, Zeta potential, VSM, and TGA analyses. The cytotoxic effect of the decorated nanocomposites on the MCF-7 cancer cell line was studied. It was found that C. Protease/DOX decorated Fe₃O₄/SiO₂ nanocomposite showed 7.5% cell viability as measured by the MTT test.

Journal of Drug Delivery Science and Technology

Volume 80, February 2023, 104144

Cladosporium protease/doxorubicin decorated Fe3O4@SiO2 nanocomposite: An efficient nanoparticle for drug delivery and combating breast cancer - ScienceDirect